Summary

OpTooGlu aims to develop and establish new optogenetic tools for temporal gene expression in bacteria and the first ones for the acetic acid bacterium and versatile industrial workhorse Gluconobacter oxydans because of its huge biotransformation potential. The ultimate goal is to establish the simultaneous use of UV-A- and green light-sensitive caged effector molecules for two-color bioprocess engineering strategies to control cellular functions in bacteria. For Gluconobacter, the new optogenetic tools developed herein will be used for the first time as a proof-of-concept in bioprocess engineering by light-mediated temporal control of gene expression to optimize expression of membrane-bound dehydrogenases for improved high-level production of the high-value compound L-arabinonic acid from renewable L-arabinose present in related biorefining biomass streams.

SEED FUND 3.0 Coordinator

Dr. Tino Polen
IBG-1 - Biotechnology
Forschungszentrum Jülich
email: t.polen[at]fz-juelich.de

Partners

Prof. Dr. Wolfgang Wiechert, Dr. Dietrich Kohlheyer, Dr. Tino Polen, IBG-1: Biotechnology | Systems Biotechnology, Forschungszentrum Jülich
Prof. Dr. Pietruszka, IBOC - Bioorganic Chemistry, HHU Düsseldorf
Prof. Dr. Thomas Drepper, IMET - Molecular Enzyme Technology, HHU Düsseldorf

Funding period

01.01.2024 - 31.12.2025

Funding

OpTooGlu is part of the NRW-Strategieprojekt BioSC and thus funded by the Ministry of Culture and Science of the German State of North Rhine-Westphalia.