29th BioSC Lecture | 10 November 2023 | Prof. Dr. Verena Siewers, Chalmers University of Technology, Gothenburg
The 29th BioSC Lecture took place on 10 November 2023 as part of the Jülich Biotech Day. Prof. Dr. Verena Siewers from Chalmers University of Technology in Gothenburg presented the establishment of yeast as a platform organism for the synthesis of flavonoids in a microbial consortium, using metabolic biosensors and directed evolution methods.
Flavonoids are plant secondary metabolites that have antioxidant, anti-inflammatory, antimutagenic or anticarcinogenic properties, depending on the subgroup. They are used, for example, in food supplements, cosmetics and pharmaceuticals. The growing demand for flavonoids is currently offset by the costly extraction of flavonoids from plants.
The SynBio4Flav project aims to synthesise flavonoids using a synthetic microbial consortium. The complex synthesis pathways are broken down into specific sections, which are introduced into the respective optimal production organism. The precursor molecules are produced by Escherichia coli and Pseudomonas putida and the end products by yeast. Challenges include the low activities of plant secondary metabolism enzymes and the toxicity of the intermediates for the microbial production organisms. Based on selected examples, Verena Siewers presented the optimisation of the synthesis sections in yeast by using metabolic biosensors and methods of directed evolution.
The co-cultivation of different microorganisms has not yet been fully achieved in the project. Nevertheless, the project's approach has great general potential for the biotechnological synthesis of complex natural substances.
More Information: https://synbio4flav.eu/