Surfactants are molecules that alter the surface tension of a liquid or the interfacial tension of liquid/liquid, gas/liquid or liquid/solid dispersions. Hence, they are most commonly used as detergents or emulsifiers. Biosurfactants are natural products of microbial origin, being biodegradable and available in a broad diversity of different variants with highly specialized attributes. However, economical production is still hampered by high raw-material costs, complex production processes, and low manufacturing output. In the spirit of a sustainable bioeconomy, the Biosurfactant Biorefinery (Bio2) project aims at the development of a biorefinery process for the production of next-generation biosurfactants rhamnolipids (RLs) and mannosylerythritol lipids (MELs) with recombinant strains of Pseudomonas putida and Ustilago maydis, respectively. Interdisciplinary cooperation of seven core groups will provide a well-founded basis to establish novel and innovative solutions addressing cutting-edge process development on multiple levels. These will include strain optimization, improvement of renewable substrate utilization, advanced fermentation concepts and process control strategies, integrated downstream processing and recycling of biomass, nutrients and water. Ultimately, the modular concept of the research biorefinery in the new NGP2 building (Center for next generation processes and products) at RWTH Aachen combined with the multidisciplinary Bio2 team provides an unmet level of flexibility and versatility, offering an ideal basis to achieve progress in the state-of-the-art of sustainable production of next generation biosurfactants.
Facing today’s grand societal challenges, two of the main goals of the NRW Research Strategy are the reduction of greenhouse gas emissions and the improvement of resource efficiency. Within the scope of the proposed project, the realization of innovative and sustainable biorefinery concepts has a high potential of contributing to both of these aspects. Bio2 will furthermore not only provide a solid scientific background for the bioeconomy, but will also enhance the integration of social, engineering, and natural sciences for a transdisciplinary assessment, taking into account process efficiency as well as sustainability criteria. Due to the wide range of potential applications for biosurfactants, structural changes in the chemical, pharmaceutical, medical, and agricultural industries may be promoted. Especially with regard to biorefineries, which represent a prioritized research topic in the NRW Bioeconomy strategy, significant new insights in process development of biosurfactant processes will be generated. The proposed project is, therefore, tailored to the current bioeconomy strategies.
|FocusLab coordinator (leader)
|Dr.-Ing. Georg Wandrey
AVT - Aachener Verfahrenstechnik
RWTH Aachen University
52074 Aachen, Germany
Phone: +49 241 80-47852
Fax: +49 241 80-22570
Julia Fritsch, M.Sc
01.05.2017 – 31.04.2020
The total budget of Bio² is 2,289,709 €. Bio² is part of the NRW-Strategieprojekt BioSC and thus funded by the Ministry of Culture and Science of the German State of North Rhine-Westphalia.
Geiser, E., Reindl, M., Blank, L.M., Feldbrügge, M., Wierckx, N., and K. Schipper. (2016). Activating intrinsic carbohydrate-active enzymes of the smut fungus Ustilago maydis for the degradation of plant cell wall components. Appl. Env. Microbiol. 82. 5174-5185.
Tiso, T., Sabelhaus, P., Behrens, B., Hayen, H., Blank, L.M. (2016). Creating metabolic demand as engineering strategy in Pseudomonas putida - Rhamnolipid synthesis as example. Metabolic Engineering Communications. 3. 234-244
Loeschcke, A., Markert, A., Wilhelm, S., Wirtz, A., Rosenau, F., Jaeger. K.-E., Drepper, T. (2013). TREX: a universal tool for the transfer and expression of biosynthetic pathways in bacteria. ACS Synthetic Biology. 2. 22-33.
Knabben, I., Regestein, L., Marquering, F., Steinbusch, S., Lara, A. R., Büchs, J. (2010). High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. J Biotechnol. 150 (1). 73-79
Abels, C., Carstensen, F., Wessling, M. (2013). Membrane processes in biorefinery applications. Journal of Membrane Science 444: 285 - 317