CombiCom Publications

2023

Bitzenhofer, NL, Classen, T, Jaeger, KE and Loeschcke, A (2023). Biotransformation Of l-Tryptophan To Produce Arcyriaflavin A With Pseudomonas putida KT2440. Chembiochem 24(23): e202300576.

Bitzenhofer, NL, Hilgers, F, Bosio, GN, Torra, J, Casini, G, Beinlich, FRM, Knieps-Grünhagen, E, Gordeliy, V, Jaeger, KE, Nonell, S, Krauss, U, Gensch, T and Drepper, T (2023). Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. Methods Mol Biol 2564: 143-183.  

Heucken, N, Tang, K, Hüsemann, L, Heßler, N, Müntjes, K, Feldbrügge, M, Göhre, V and Zurbriggen, MD (2023). Engineering and Implementation of Synthetic Molecular Tools in the Basidiomycete Fungus Ustilago maydis. Journal of Fungi 9(4): 480.  

Kossmann, DF, Huang, M, Weihmann, R, Xiao, X, Gätgens, F, Weber, TM, Brass, HUC, Bitzenhofer, NL, Ibrahim, S, Bangert, K, Rehling, L, Mueller, C, Tiso, T, Blank, LM, Drepper, T, Jaeger, K-E, Grundler, FMW, Pietruszka, J, Schleker, ASS and Loeschcke, A (2023). Production of tailored hydroxylated prodiginine showing combinatorial activity with rhamnolipids against plant-parasitic nematodes. Frontiers in Microbiology 14.

2022

Brehl, C, Brass, HUC, Lüchtrath, C, Böckmann, L, Ihling, N, Classen, T, Pietruszka, J and Büchs, J (2022). Optimized prodigiosin production with Pseudomonas putida KT2440 using parallelized noninvasive online monitoring. Biotechnol Prog 38(3): e3245.

Hilgers, F, Hogenkamp, F, Klaus, O, Kruse, L, Loeschcke, A, Bier, C, Binder, D, Jaeger, K-E, Pietruszka, J and Drepper, T (2022). Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers. Frontiers in Bioengineering and Biotechnology 10.  

Hogenkamp, F, Hilgers, F, Bitzenhofer, NL, Ophoven, V, Haase, M, Bier, C, Binder, D, Jaeger, K-E, Drepper, T and Pietruszka, J (2022). Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems. ChemBioChem 23(1): e202100467.  

Johann, S, Weichert, FG, Schröer, L, Stratemann, L, Kämpfer, C, Seiler, T-B, Heger, S, Töpel, A, Sassmann, T, Pich, A, Jakob, F, Schwaneberg, U, Stoffels, P, Philipp, M, Terfrüchte, M, Loeschcke, A, Schipper, K, Feldbrügge, M, Ihling, N, Büchs, J, Bator, I, Tiso, T, Blank, LM, Roß-Nickoll, M and Hollert, H (2022). A plea for the integration of Green Toxicology in sustainable bioeconomy strategies – Biosurfactants and microgel-based pesticide release systems as examples. Journal of Hazardous Materials 426: 127800.  

Klaus, O, Hilgers, F, Nakielski, A, Hasenklever, D, Jaeger, K-E, Axmann, IM and Drepper, T (2022). Engineering phototrophic bacteria for the production of terpenoids. Current Opinion in Biotechnology 77: 102764.  

Weihmann, R, Kubicki, S, Bitzenhofer, NL, Domröse, A, Bator, I, Kirschen, L-M, Kofler, F, Funk, A, Tiso, T, Blank, LM, Jaeger, K-E, Drepper, T, Thies, S and Loeschcke, A (2022). The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida. FEMS Microbes 4. 

2021

Hogenkamp, F., et al. (2021). "Effect of Photocaged Isopropyl β-d-1-thiogalactopyranoside Solubility on the Light Responsiveness of LacI-controlled Expression Systems in Different Bacteria." Chembiochem : a European journal of chemical biology 22(3): 539-547.

2020

Baum, C. M. and B. Bartkowski (2020). "It’s not all about funding: Fostering interdisciplinary collaborations in sustainability research from a European perspective." Energy Research & Social Science 70: 101723

Brands S, Brass HUC, Klein AS, Pietruszka J, Ruff AJ, and Schwaneberg U (2020) A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC. Chem. Commun. doi:10.1039/D0CC02181D.

Butkowski OK, Baum CM, Pakseresht A, Bröring S, Lagerkvist CJ (2020) Examining the social acceptance of genetically modified bioenergy in Germany: Labels, information valence, corporate actors, and consumer decisions. Energy Research & Social Science 60:101308. doi: 10.1016/j.erss.2019.101308

Fejzagić, A. V., et al. (2020). "A Fluorescence-Based Assay System for the Determination of Haloperoxidase-Activity Using a Two-Dimensional Calibration Ap-proach." ChemistryOpen 9(9): 959-966.

Habash, S. S., et al. (2020). "Novel Prodiginine Derivatives Demonstrate Bioactivities on Plants, Nematodes, and Fungi." Front Plant Sci 11: 579807-579807.
   
Habash, S. S., et al. (2020). "The Plant Sesquiterpene Nootkatone Efficiently Reduces Heterodera schachtii Parasitism by Activating Plant Defense." Int J Mol Sci 21(24): 9627.
    
Harwoko, H., et al. (2020). "Azacoccones F-H, new flavipin-derived alkaloids from an endophytic fungus Epicoccum nigrum MK214079." Fitoterapia 146: 104698.

Lee J, Hilgers F, Loeschke A, Jaeger K-E, and Feldbrügge M (2020) Ustilago maydis serves as a novel production host for the synthesis of plant and fungal sesquiterpenoids. Front. Microbiol., doi: 10.3389/fmicb.2020.01655

Lee, J., et al. (2020). "Identification of Feldin, an Antifungal Polyyne from the Beefsteak Fungus Fistulina hepatica."  10(11): 1502.

Loeschcke A, Thies S (2020) Engineering of natural product biosynthesis in Pseudomonas putida. Curr Opin Biotechnol. 65:213–224.

Müntjes K, Philipp M, Hüsemann L, Heucken N, Weidtkamp-Peters S, Schipper K, Zurbriggen MD and Feldbrügge M (2020) Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis. Front. Microbiol. 11:1384.

Pan F, EI-Kashef D, Kalscheuer R, Müller WEG, Lee J, Feldbrügge M, Mándi A, Kurtán T, Liu Z, Wu W, Proksch P (2020) Cladosins L-O, new hybrid polyketides from the endophytic fungus Cladosporium sphaerospermum WBS017. Eur. J. Med. Chem. 191: 113159.

Weihmann R, Domröse A, Drepper T, Jaeger KE, Loeschcke A (2020) Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida. Microb Biotechnol. 13(1):250-262.

2019

Baum CM (2019). When Technology and Reality Meet: Exploring the Potential of a Constructive Precautionary Principle for Genome-Editing Technologies. In C. Dürnberger, S. Pfeilmeier, & S. Schleissing (Eds.), Genome Editing in Agriculture. Between Precaution and Responsibility (pp. 197-211). TTN-Studien Volume 7. Baden-Baden: Nomos. ISBN: 978-3-8487-5518-9.

Bartkowski, B. & Baum C.M. (2019) Dealing With Rejection: An Application of the Exit–Voice Framework to Genome-Edited Food. Frontiers in Bioengineering and Biotechnology 7:57. doi: 10.3389/fbioe.2019.00057.

Brass HUC, Klein AS, Nyholt S, Classen T, Pietruszka J (2019) Condensing Enzymes from Pseudoalteromonadaceae for Prodiginine Synthesis. Adv. Synth. Catal.361(11):2659-2667.

Burmeister, A., et al. (2019). "A microfluidic co-cultivation platform to investigate microbial interactions at defined microenvironments." Lab on a Chip 19(1): 98-110.

Domröse A, Hage-Hülsmann J, Thies S, Weihmann R, Kruse L, Otto M, Wierckx N, Jaeger KE, Drepper T, Loeschcke A (2019) Pseudomonas putida rDNA is a favored site for the expression of biosynthetic genes. Sci Rep. 9(1):7028.

Fejzagić AV, Gebauer J, Huwa N, Classen T (2019) Halogenating Enzymes for Active Agent Synthesis: First Steps Are Done and Many Have to Follow. Molecules 24(21):4008.

Hage-Hülsmann J, Metzger S, Wewer V, Buechel F, Troost K, Thies S, Loeschcke A., Jaeger K-E, Drepper T (2019) Biosynthesis of cycloartenol by expression of plant and bacterial oxidosqualene cyclases in engineered Rhodobacter capsulatus. J Biotechnol X 4: 100014.

Harwoko H, Daletos G, Stuhldreier F, Lee J, Wesselborg S, Feldbrügge M, Müller WEG, Kalscheuer R, Ancheeva E, Proksch P (2019) Dithiodiketopiperazine derivatives from endophytic fungi Trichoderma harzianum and Epicoccum nigrum. Nat. Prod. Res. 18:1-9.

Hilgers, F., et al. (2019). "Genetically Encoded Photosensitizers as Light-Triggered Antimicrobial Agents." Int J Mol Sci 20(18): 4608

Kallscheuer N, Classen T, Drepper T, Marienhagen J (2019) Production of plant metabolites with applications in the food industry using engineered microorganisms. Curr Opin Biotechnol. 56:7-17.

Nogueira M, Enfissi EMA, Welsch R, Beyer P, Zurbriggen MD, Fraser PD (2019) Construction of a fusion enzyme for astaxanthin formation and its characterization in microbial and plant hosts: A new tool for engineering ketocarotenoids. Metabolic Engineering 52:243-252. doi: 10.1016/j.ymben.2018.12.006.

Otto M, Wynands B, Drepper T, Jaeger K-E, Thies S, Loeschcke A, Blank LM, Wierckx N (2019). Targeting 16S rDNA for Stable Recombinant Gene Expression in Pseudomonas. ACS Synth Biol 8(8):1901 – 1912.

Troost K, Loeschcke A, Hilgers F, Özgür AY, Weber TM, Santiago-Schübel B, Svensson V, Hage-Hülsmann J, Habash SS, Grundler FMW, Schleker S, Jaeger K-E, Drepper T (2019). Engineered Rhodobacter capsulatus as a phototrophic platform organism for the synthesis of plant sesquiterpenoids. Front Microbiol. 10: 1998.

2018

Baaske J, Gonschorek P, Engesser R, Dominguez-Monedero A, Raute K, Fischbach P, Müller K, Cachat E, Schamel WWA, Minguet S, Davies J, Timmer J, Weber W, Zurbriggen MD. (2018). Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Scientific Reports. 8(1):15024.

Beyer HM, Engesser R, Hörner M, Koschmieder J, Beyer P, Timmer J, Zurbriggen MD, Weber W (2018) Synthetic Biology Makes Polymer Materials Count. Advanced Materials 30(21):e1800472

Beyer HM, Thomas OS, Riegel N, Zurbriggen MD, Weber W, Hörner M (2018) Generic and reversible opto-trapping of biomolecules. Acta Biomaterialia 79: 276-282.

Chatelle C, Ochoa-Fernandez R, Engesser R, Schneider N, Beyer HM, Jones AR, Timmer J, Zurbriggen MD, Weber W (2018). A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells. ACS Synthetic Biology 7(5):1349-1358.

Classen T, Pietruszka J (2018) Complex molecules, clever solutions - enzymatic approaches towards natural product and active agent syntheses. Bioorg Med Chem. 26(7):1285-1303.

Endres S, Wingen M, Torra J, Ruiz-González R, Polen T, Bosio G, Bitzenhofer NL, Hilgers F, Gensch T, Nonell S, Jaeger K-E, Drepper T (2018) An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria. Scientific Reports 8(1):15021.

Hage-Hülsmann J, Grünberger A, Thies S, Santiago-Schübel B, Klein AS, Pietruszka J, Binder D, Hilgers F, Domröse A, Drepper T, Kohlheyer D, Jaeger K-E, Loeschcke A (2018). Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants. PLoS ONE 13(7): e0200940.

Klein AS, Brass HUC, Klebl DP, Classen T, Loeschcke A, Drepper T, Sievers S, Jaeger K-E, Pietruszka J (2018) Preparation of cyclic prodiginines by mutasynthesis in Pseudomonas putida KT2440. ChemBioChem 19(14):1545-1552.

2017

Domröse A, Weihmann R, Thies S,  Jaeger K-E, Drepper T, Loeschcke A (2017) Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX, Synth Syst Biotechnol. 2(4):310-319.

Klein AS, Domröse A, Bongen P, Brass HUC, Classen T, Loeschcke A, Drepper T, Laraia L, Sievers S, Jaeger KE, Pietruszka J (2017). New Prodigiosin Derivatives Obtained by Mutasynthesis in Pseudomonas putida. ACS Synthetic Biology 6(9):1757–1765.

Loeschcke A, Dienst D, Wewer V, Hage-Hülsmann J, Dietsch M, Kranz-Finger S, Hüren V, Metzger S, Urlacher VB, Gigolashvili T, Kopriva S, Axmann IM, Drepper T, Jaeger K-E (2017) The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS ONE 12(12):e0189816.