Bioeconomy Science Center
Forschung und Kooperation für nachhaltige BioÖkonomie

Bioeconomy Science Center
Forschung und Kooperation für nachhaltige BioÖkonomie
SEED FUND Projekt FlowCom

Everything in Flow – New Compartments for Cascade Reaction

Results

The project aims at synthesizing protein/enzyme-based active membranes to be employed in membrane modules for the efficient process control of aldolase-catalyzed reactions. This modular approach is based on the synthesis of protein-polymer conjugates, which form a thin nanoporous membrane via self-assembly at fluid interfaces. The membrane function is controlled via the protein/enzyme building block employed. Membrane stability will be introduced via the proper choice of the polymer matrix.

Participating Core Groups (CG)

Coordinator
Prof. Dr. A. Böker
, Makromolekulare Materialien und Oberflächen (MMO), RWTH Aachen University
Forckenbeckstr. 50
52056 Aachen
Germany
phone: +49 241 80 23304
e-mail: boker@dwi.rwth-aachen.de

Partners
Prof. Dr. J. Pietruszka
, Bioorganische Chemie (BOC), Heinrich-Heine-University Düsseldorf
Prof. Dr. A. Spieß, AVT – Enzymprozesstechnik (AVT – EPT), RWTH Aachen University


Funding period

01.07.2014 – 30.09.2015


Funding
The total budget of FlowCom is € 149.039. FlowCom is part of the NRW-Strategieprojekt BioSC and thus funded by the Ministry of Innovation, Science and Research of the German State of North Rhine-Westphalia.


Publications

Bisterfeld, C, Küberl, I, Dick, M and Pietruszka, J (2016). A fluorogenic screening for enantio- and diastereoselectivity of 2-deoxy-d-ribose-5-phosphate aldolases. Synlett 27(01): 11-16.

Dick, M, Hartmann, R, Weiergraber, OH, Bisterfeld, C, Classen, T, Schwarten, M, Neudecker, P, Willbold, D and Pietruszka, J (2016). Mechanism-based inhibition of an aldolase at high concentrations of its natural substrate acetaldehyde: Structural insights and protective strategies. Chemical Science 7(7): 4492-4502.

Dick, M, Weiergräber, OH, Classen, T, Bisterfeld, C, Bramski, J, Gohlke, H and Pietruszka, J (2016). Trading off stability against activity in extremophilic aldolases. Scientific Reports 6: 17908.

Reinicke, S, Rees, HC, Espeel, P, Vanparijs, N, Bisterfeld, C, Dick, M, Rosencrantz, RR, Brezesinski, G, de Geest, BG, Du Prez, FE, Pietruszka, J and Boker, A (2017). Immobilization of 2-deoxy-d-ribose-5-phosphate aldolase in polymeric thin films via the langmuir-schaefer technique. ACS Appl Mater Interfaces 9(9): 8317-8326